Our goals were to 1) isolate, and culture two fungal morphotypes, 2) characterize the volatile emissions from grain inoculated by each fungal morphotype (Aspergillus flavus or Fusarium spp.) compared to uninoculated and sanitized grain, and 3) understand how MVOCs from each morphotype affects mobility, attraction, and preference by L. serricorne. Headspace collection revealed that the Fusarium- and A. flavus-inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with A. flavus compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to L. serricorne at a longer distances in commercial pitfall traps. While there was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer, it is possible that methodological limitations prevented robust interpretation from this assay. Overall, our study suggests that MVOCs are important for close- and long-range orientation of L.serricorne during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species.

This dataset is associated with the forthcoming publication entitled, "Microbial volatile organic compounds mediate attraction by a primary but not secondary stored product insect pest in wheat", and includes data on grain damage from near infrared spectroscopy, behavioral data from wind tunnel and release-recapture experiments, as well as volatile characterization of headspace from moldy grain. For all files, incubation intervals 9, 18, and 27 d represent how long grain was incubated after being tempered to a grain moisture of 12, 15, or 19% or left untempered (ctrl; 10.8% grain moisture). TSO = Trece storgard oil; empty = negative control (no stimulus), LGB = lesser grain borer (Rhzyopertha dominica), and RFB = red flour beetle (Tribolium castaneum). Note: The resource 'GC/MS Grain MVOC Headspace Data' was added 2021-08-04 with the deletion of some compounds as unlikely natural compounds and potential contaminants. This is the dataset that undergirds the non-metric multidimensional scaling analysis. See the included file list for more information about methods and results of each file in this dataset.

Data collected by Sabita Ranabhat from early Sep 2020 - Jun 2021. It consists of two experiments: one evaluating mobility of Tribolium castaneum and Rhyzopertha dominica after exposure to differently formulated controlled release materials for 0-60 min, and a second experiment looking at median lethal time after exposure to cheesecloth treated with permethrin, deltamethrin, indoxacarb, and dinotefuran between 0-168 h. Tested strains include phosphine-resistant and phosphine-susceptible populations.

Datasets archived here consist of all data analyzed in Duan et al. 2015 from Journal of Applied Ecology. Specifically, these data were collected from annual sampling of emerald ash borer (Agrilus planipennis) immature stages and associated parasitoids on infested ash trees (Fraxinus) in Southern Michigan, where three introduced biological control agents had been released between 2007 - 2010. Detailed data collection procedures can be found in Duan et al. 2012, 2013, and 2015.