The data provided is from a study on overwintering honey bee colonies using cold storage. Colonies were summered in two different geographic regions of the USA: South Texas and North Dakota. Colonies summered in North Dakota were placed in cold storage in either October or November of 2019. Placement in cold storage of hives summered in South Texas happened in November of 2019. A second set of colonies overwintered in south Texas. All sets of colonies were evaluated and sampled prior to and after cold storage and again after almond bloom in 2020. Colonies overwintered in South Texas were evaluated during the same periods as those in cold storage. Data are provided for frames of bees and brood pre and post cold storage as well as after almond bloom. The data included in the two files shows: bee frame counts, mite counts and brood counts. Lab data provided shows fat body weight as well as protein and lipid concentrations in worker bees pre and post cold storage /overwintering. Resources in this dataset: Resource Title: Colony Evaluations Pre and Post Cold Storage File Name: colony sizes.xlsx Resource Description: The data shows the colony evaluations including frames of brood, frames of bees, and pre treatment mite counts pre and post cold storage. Resource Title: Protein and Lipid Concentrations of Honey Bees Colonies Pre and Post Cold Storage File Name: fat body col sizes and analysis.xlsx Resource Description: The data shows the pre and post cold storage colony lipid and protein concentrations. Data also shows the average number frames of bees, brood and mite counts associated with these treatments.