The study of ecological networks along environmental gradients has so far been limited by the difficulty of collecting large-scale dataset of comparable interactions. Here, we compiled 48 plant–orthoptera interaction networks at multiple locations across the Swiss Alps (i.e. along six elevation gradient). Trophic interactions were obtained by applying next-generation sequencing methods (e.g. DNA metabarcoding) on insect feces. Together with interaction data, we also provide data of the functional trait measurement (i.e. plant leaves traits and insect mandibular strength) expected to influence the realization of the interaction. Species inventories, feces samples and functionals traits were collected during the summer 2016 and 2017. Lab work and network reconstruction were completed in 2019.