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Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments
OwnerUK Centre for Ecology & Hydrology (UKCEH) - view all
Update frequencyunknown
Last updated26 days ago
Overview

This dataset contains operational taxonomic units for epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Additional Information
KeyValue
contact_emailenquiries@ceh.ac.uk
contact_nameGriffiths, R.
contact_urihttps://catalogue.ceh.ac.uk/id/16649ff0-af24-41b0-bcb4-15e610dac170_c0
dcat_typehttp://purl.org/dc/dcmitype/Dataset
guidhttps://catalogue.ceh.ac.uk/id/16649ff0-af24-41b0-bcb4-15e610dac170
identifier1395928899368
language["eng"]
provenancen2e3fb7e86ad3492f87da893fc97bb5beb8902
publisher_urihttps://ror.org/04xw4m193
spatial_textPOLYGON((-6.452 49.864, -6.452 55.812, 1.768 55.812, 1.768 49.864, -6.452 49.864))
urihttps://catalogue.ceh.ac.uk/id/16649ff0-af24-41b0-bcb4-15e610dac170
harvest_object_idc4d24837-80e8-4d8a-9f86-c0597fb467cf
harvest_source_idd4fbf67d-0e8f-4732-a34e-be92ef65e401
harvest_source_titleceh-eidc
Files
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    077a4d42-124c-4897-a50c-b7365c7ed05f
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