The dataset includes mean +/- standard deviation of each experiment ex vitro (in vitro) rat, in vivo rat, ex vitro (in vitro) human, calculated human. The rows show each fraction or factor in the experiment (e.g., skin wash, tape strip, etc.). The ex vivo data is from work completed at EPA-RTP. The in vivo data was from work completed at NIEHS. This dataset is associated with the following publication: Knudsen, G., A. Trexler, A. Richiards, M. Hughes, and L. Birnbaum. 2,4,6-Tribromophenol disposition and kinetics in rodents: effects of dose, route, sex, and species. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 169(1): 167-179, (2019).