The supplementary data is used to support the interpretation of electrochemical reaction at the interface of working electrode, and reaction features of the immobilized enzyme. The formula for modeling the kinetics of immobilized enzyme was also given in the supplementary material. This dataset is associated with the following publication: Li, T., E. Sahle-Demessie, E. Varughese, and J. Berberich. A disposable acetylcholine esterase sensor for As(iii) determination in groundwater matrix based on 4-acetoxyphenol hydrolysis. Analytical Methods. RSC Publishing, Cambridge, UK, 11(40): 5203-5213, (2019).