Dataset for Phelps, et al. "A high-throughput chemotaxis assay for assessing immunotoxicity using multiple human immune cell types", published in NAM Journal, Volume 1, Nov2025 (https://doi.org/10.1016/j.namjnl.2025.100066). Supplementary figures and tables are available here: https://ars.els-cdn.com/content/image/1-s2.0-S3050620425000612-mmc1.pdf . All raw data corresponding to the manuscript is available here: https://ars.els-cdn.com/content/image/1-s2.0-S3050620425000612-mmc2.xlsx . This dataset is associated with the following publication: Phelps, D., N. Barbo, S. Caty, J. Harrill, and K. Slentz-Kesler. A high-throughput chemotaxis assay for assessing immunotoxicity using multiple human immune cell types. NAM Journal. Elsevier B.V., Amsterdam, NETHERLANDS, 1: 100066, (2025).