The majority of this dataset includes the query output from online databases ECOTOX and SeqAPASS used to support the ecological AOP case studies described within the manuscript. The final worksheet includes the raw data used to generate concentration vs response curves for four putative chemical initiators (Supplemental Figure S4). This dataset is associated with the following publication: Fay, K., D. Villeneuve, C. LaLone, Y. Song, K.E. Tollefsen, and G. Ankley. Practical approaches to adverse outcome pathway (AOP) development as illustrated by ecological case studies. SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY JOURNAL. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 36(6): 1429–1449, (2017).