Raw data associated with the manuscript titled Application of Transcriptomics Concentration-Response Modeling for Prioritization of Contaminants Detected in Tributaries of the North American Great Lakes (Cavallin et al., 2025). This dataset is associated with the following publication: Cavallin, J., K. Bush, S. Corsi, L. DeCicco, K. Flynn, A. Kasparek, M. Hazemi, E. Maloney, P. Schumann, and D. Villeneuve. Application of transcriptomics concentration-response modeling for prioritization of contaminants detected in tributaries of the North American Great Lakes. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 44(5): 1310-1321, (2025).