Dataset includes data from each experiment conducted in the pilot-scale testing. Each sheet of the Excel file pertains to each test. A data dictionary is included in the first sheet. In each sheet there are microbiological data (colony forming units) for each test and positive control coupon used in the study. Also shown is the calculation of decontamination efficacy (log10 reduction). This dataset is associated with the following publication: Wood, J., W. Calfee, S. Ryan, L. Mickelsen, M. Clayton, and V. Rastogi. A Simple Decontamination Approach Using Hydrogen Peroxide Vapor for Bacillus anthracis Spore Inactivation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(6): 1603-1615, (2016).