This data set describes changes in concentrations of somatic and F+ coliphages and adenovirus in freshwater under the influence of different biotic and abiotic factors. This dataset is associated with the following publication: McMinn, B., E. Rhodes, E. Webster, and A. Korajkic. Decay of infectious adenovirus and coliphages in freshwater habitats is differentially affected by ambient sunlight and the presence of indigenous protozoa communities. JOURNAL OF VIROLOGY. American Society for Microbiology, Washington, DC, USA, 17: 1, (2020).