Supplementary data for "Derivation and characterization of environmental hazard concentrations for chemical prioritization: a case study in the Great Lakes tributaries". This dataset is associated with the following publication: Maloney, E., S. Corsi, M. Pronschinske, L. DeCicco, M. Nott, J. Frisch, N. Fuller, A. Baldwin, K. Kimbrough, M. Edwards, S. Hummel, N. Vinas, and D. Villeneuve. Derivation and characterization of environmental hazard concentrations for chemical prioritization: a case study in the Great Lakes tributaries. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 44(7): 2029-2047, (2025).