This dataset includes the standard curves for ICCRTqPCR to convert the assay quantities to the concentrations of infectious viruses and all the calculations on inactivation rate constants. Also, all the figures used in the manuscripts are presented. This dataset is associated with the following publication: Ryu, H., K. Schrantz, N. Brinkman, and L. Boczek. Applicability of integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) for the simultaneous detection of the four human enteric enterovirus species in disinfection studies. JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 258: 35-40, (2018).