The data is in the form of genomic sequences deposited in a public database, growth curves, and bioinformatic analysis of sequences. This dataset is associated with the following publication: Henson, M., J. Santodomingo , P. Kourtev, R. Jensen, and D. Learman. Metabolic and genomic analysis elucidates strain-level variation in Microbacterium spp. isolated from chromate contaminated sediment. PeerJ. PeerJ Inc., Corte Madera, CA, USA, e1395, (2015).