Dataset includes the SRA accessions of the sequencing data used to benchmark the MetaCompare 2.0 pipeline as well as tables of bacterial taxa and antibiotic resistance genes used to perform the risk assessments. A link to the GitHub page where the pipeline source code can be found is also provided. This dataset is associated with the following publication: Rumi, M., M. Oh, B. Davis, C. Brown, J. Adeesh, P. Vikesland, A. Pruden, and L. Zhang. MetaCompare 2.0: Differential ranking of ecological and human health resistome risks. FEMS Microbiology Ecology. Oxford University Press, OXFORD, UK, 100(12): fiae155, (2024).