Multivariate and univariate datasets published in associated manuscript that describes the application of metabolomics for non-lethal monitoring of sturgeon species. This dataset is associated with the following publication: Collette, T., S. Romano, Q. Teng, A. Fox, S. Kornberg, and D. Ekman. Evaluation of minimally invasive metabolomic methods for assessing the health of sturgeons. Scientific Reports. Nature Publishing Group, London, UK, 15: 34461, (2025).