This file contains the data used to generate hit calls from neural activity recordings on microelectrode array (MEA) plates treated with ToxCast compounds at a single concentration. This dataset is associated with the following publication: Strickland, J., M. Martin, A. Richard, K. Houck, and T. Shafer. Screening the ToxCast phase II libraries for alterations in network function using cortical neurons grown on multi-well microelectrode array (mwMEA) plates. Archives of Toxicology. Springer, New York, NY, USA, 92(1): 487-500, (2018).