The dataset consists of daily measurements of N2O, N2O isotopic abundance and site preference, and CO2 flux. Data are presented as a daily averages of 10 second data, obtained over a 46 day period. This dataset is associated with the following publication: Yuan, Y., H. Chen, W. Yuan, D. Williams, J. Walker, and w. Shi. Is biochar-manure co-compost a better solution for soil health improvement and N2O emissions mitigation?. BIOGEOCHEMISTRY. Springer, New York, NY, USA, 113: 14-25, (2017).