These data are published in a manuscript entitled Multi-tissue transcriptomic and serum metabolomic assessment reveals systemic implications of acute ozone-induced stress response in male Wistar Kyoto rats in the journal Metabolomics. This analyses explored systemic transcriptomic and circulating metabolomic effects of inhaled ozone exposure in rats. This dataset is associated with the following publication: Jackson, T., J. House, A. Henriquez, M. Schladweiler, K.M. Jackson, A. Astriab Fisher, S. Snow , D. Alewel, A. Motsinger-Reif, and U. Kodavanti. Multi-tissue transcriptomic and serum metabolomic assessment reveals systemic implications of acute ozone-induced stress response in male Wistar Kyoto rats. Metabolomics. Plenum Press, New York, NY, USA, 19: 81, (2023).