This data set contains variables measured during a survey of sediment-methane production rates in a eutrophic reservoir in southwestern Ohio. The measured production rates and a suite of predictor variables including water chemistry, sediment characteristics, and algal abundance are presented.. This dataset is associated with the following publication: Berberich, M., J. Beaulieu, T. Hamilton, S. Waldo, and I. Buffam. Spatial variability of sediment methane production and methanogen communities within a eutrophic reservoir: importance of organic matter source and quantity. LIMNOLOGY AND OCEANOGRAPHY. American Society of Limnology and Oceanography, Lawrence, KS, USA, 65(3): 1-23, (2020).