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Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: CO1 dataset
L o a d i n g
Organization
United State Environmental Protection Agency - view all
Update frequencyunknown
Last updated4 days ago
Format
Overview

Fecal samples from migratory birds were collected from contaminated wetlands in the Bunker Hill Lower Basin Coeur ’d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected. CO1 primers with Illumina adaptors: CO1-F TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAACATTCAGCCATCTTACCCGTG CO1-R GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACAGGGAGTGATAGRAGKAGTAGG Also, chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds, which can be found at DOI: 10.23719/1532393

Amplicon-sequencingBirdsCO1Pb toxicity
Additional Information
KeyValue
Dcat Modified2025-06-26
Dcat Publisher NameU.S. EPA Office of Research and Development (ORD)
Guidhttps://doi.org/10.23719/1532394
Harvest Object Idfbc11b9a-338d-49e6-b127-e5f1388d5678
Harvest Source Idb8e63f83-bbb9-45d3-a3de-09607cc9ff8a
Harvest Source TitleUSEPA Environmental Dataset Gateway
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Trust Framework(s)None
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Data Sensitivity Classunknown
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Files
  • https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348

  • Bird_CO1_SRA_metadata_Final.xlsx