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Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: rbcL dataset
L o a d i n g
Organization
United State Environmental Protection Agency - view all
Update frequencyunknown
Last updated4 days ago
Format
Overview

Fecal samples from migratory birds were collected from Pb contaminated wetlands in the Bunker Hill Lower Basin Coeur ’d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds. rbcL primers with Illumina adaptors: RBCL-F TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGYGATGGACTTACNAGTCTTGATCGTTACAAAGG RBCL-R GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGNCCATAYTTRTTCAATTTATCTCTTTCAACTTGGATNCC Also, mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected, which can be found at DOI: 10.23719/1532394

Amplicon-sequencingBirdsPb toxicityrbcL
Additional Information
KeyValue
Dcat Modified2025-06-26
Dcat Publisher NameU.S. EPA Office of Research and Development (ORD)
Guidhttps://doi.org/10.23719/1532393
Harvest Object Id13820b62-9a8b-4bc5-b01b-04c76af38949
Harvest Source Idb8e63f83-bbb9-45d3-a3de-09607cc9ff8a
Harvest Source TitleUSEPA Environmental Dataset Gateway
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Files
  • https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1277348

  • Bird_rbcL_SRA_metadata_Final.xlsx