Using fecal DNA metabarcoding to test if diet of migratory birds is correlated with Pb exposure level at a contaminated site in north Idaho: rbcL dataset
L o a d i n g
Organization
United State Environmental Protection Agency - view all
Update frequencyunknown
Last updated4 days ago
OverviewAmplicon-sequencingBirdsPb toxicityrbcL
Fecal samples from migratory birds were collected from Pb contaminated wetlands in the Bunker Hill Lower Basin Coeur ’d Alene River Watershed and from a Hepton Lake reference area St. Joe River Watershed during 2022-2024. Chloroplast rbcL metabarcoding was used to identify plant taxa recently consumed by the birds. rbcL primers with Illumina adaptors: RBCL-F TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGYGATGGACTTACNAGTCTTGATCGTTACAAAGG RBCL-R GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGNCCATAYTTRTTCAATTTATCTCTTTCAACTTGGATNCC Also, mitochondrial CO1 metabarcoding was used to confirm bird taxa from which samples were collected, which can be found at DOI: 10.23719/1532394
Additional Information
KeyValue
Dcat Modified2025-06-26
Dcat Publisher NameU.S. EPA Office of Research and Development (ORD)
Guidhttps://doi.org/10.23719/1532393
Harvest Object Id13820b62-9a8b-4bc5-b01b-04c76af38949
Harvest Source Idb8e63f83-bbb9-45d3-a3de-09607cc9ff8a
Harvest Source TitleUSEPA Environmental Dataset Gateway
