Water stable isotope data (hydrogen and oxygen isotopes of H2O) from wetlands and streams within the Pipestem Creek watershed. This data was collected over two open water seasons (May-September): 2014 and 2015. This dataset is associated with the following publication: Brooks, J.R., D. Mushet, M. Vanderhoof, S. Leibowitz, J. Christensen, B. Neff, D. Rosenberry, W. Rugh, and L. Alexander. Estimating wetland connectivity to streams in the Prairie Pothole Region: an isotopic and remote sensing approach. WATER RESOURCES RESEARCH. American Geophysical Union, Washington, DC, USA, 54(2): 995-977, (2018).