Disinfection of B. globigii spores (a non-pathogenic surrogate for B. anthracis) in clean and dirty water using the ATS-Waterline system, which uses ultraviolet light and a charged membrane filter. This dataset is associated with the following publication: Silva, G., J. Szabo, V. Namboodiri, R. Krishnan, J. Rodriguez, and A. Zeigler. Evaluation of and environmentally sustainable UV-assisted water treatment system for removal of Bacillus spores in water. WATER SCIENCE AND TECHNOLOGY. IWA Publishing, London, UK, 18(3): 968-975, (2018).