This submission contains DNA tracer data that supports the analysis and conclusions of the publication, "DNA tracer transport through porous media -The effect of DNA length and adsorption." https://doi.org/10.1029/2020WR028382. This experiment used DNA as an artificial reservoir tracer. Groundwater tracing is an effective way to identify fluid flow pathways and estimate hydrogeologic properties, which are important premises for building reliable hydrological models for transport predictions or contamination mitigations. The objective of this study was to understand the effect of DNA length (i.e., number of base pairs for dsDNA) and adsorption on DNA transport.