The dataset contains individual genotypes at 11 nuclear microsatellite markers of samples of Swiss stone pine (*Pinus cembra*). Samples were collected in 12 natural and presumably planted stands in the canton of Fribourg (Switzerland). The dataset is complemented by individual genotypes (same markers) from samples of a subset of 40 populations across the European Alps (taken from Gugerli et al., Journal of Biogeography 2023; https://doi.org/10.1111/jbi.14586; dataset archived at Dryad, https://doi.org/10.5061/dryad.866t1g1v6).