This dataset contains biodiversity data focussing on invertebrates (soil, ground, tree canopy), and plants collected from The Carbon Community woodland creation experiment in Wales. This includes measurements from 72 plots, covering experimental treatment combinations of woodland type (native broadleaf vs sitka spruce), enhanced weathering (with/without basalt addition), and soil microbiome inoculation (with/without soil from mature habitat type), compared to a grassland control treatment. The experiment was established in April 2021 and aims to assess strategies for maximising woodland carbon storage. The biodiversity data from each experimental plot was collected in summer 2023.