This dataset contains weather conditions, water quality, water chemistry and crustacean zooplankton counts sampled at Loch Leven throughout the year 2020. Loch Leven is a lowland lake in Scotland, United Kingdom. The data were collected as part of a long-term monitoring programme, which began in 1968 and is still underway. Sampling occurs roughly every 2 weeks with laboratory analysis and data processing being performed at the UK Centre for Ecology & Hydrology Edinburgh site. The sampling and processing was supported by the Natural Environment Research Council award number NE/R016429/1 as part of the UK-SCAPE programme delivering National Capability.