The data set contains fluxes of nitrous oxide (N2O) from Whim peatbog in central Scotland, measured using chamber methods. A nitrogen deposition experiment was carried out at the site, where nitrogen was applied in different forms (ammonium, nitrate, and ammonia) at doses from zero to ~100 kg N per ha per year. Flux measurements were made over a six month period starting at the end of March, 2012. The experiment was begun in 2002 under the NERC Global Nitrogen Enrichment (GaNE) programme, and continued under various funding sources. This work was supported by the Natural Environment Research Council award number NE/R016429/1 as part of the UK-SCAPE programme delivering National Capability.