This dataset contains soil biogeochemical data collected along gradients of windthrow disturbance in Kielder Forest, Northumberland, England, in summer 2022 as part of the Monitoring Underground Disturbance (MUD) BioBank. We established ~200 m transects at 10 sites (defined by common stand type, topography) and established 50m2 sampling plots in three different disturbance regimes across each transect. Soils were analysed for moisture content, organic carbon and nitrogen, and root biomass.