This data set includes records of soil fauna sampled from the Sourhope experimental site in July and October 2002, and April, June and October 2003. These include collembola, spiders, slugs and beetles and a range of invertebrates captured by a variety of methods. Data were collected during a project funded under the NERC Soil Biodiversity Programme. The NERC Soil Biodiversity Thematic Programme was established in 1999 and was centred upon the intensive study of a large field experiment located at the Macaulay Land Use Research Institute (now the James Hutton Institute) farm at Sourhope in the Scottish Borders (Grid reference: NT8545019630). During the experiment, the site was monitored to assess changes in above-ground biomass production (productivity), species composition and relative abundance (diversity).