This data set includes counts of soil meso-fauna collected from topsoil within a wide range of land use types across Wales, collected as part of the Glastir Monitoring and Evaluation Programme (GMEP). Meso-fauna include collembola (springtails) and acari (mites). The monitoring programme was set up by the Welsh Government in 2013 to monitor the effects of the Glastir agri-environment scheme on the environment and ran from 2013 to 2016. The field survey element was based on a stratified random sampling design of 300 x 1km square sites across Wales, and was managed by the Centre for Ecology & Hydrology.