Data file for "Vliet SMF, Hazemi M, Blatz D, Jensen M, Mayasich S, Transue TR, Simmons C, Wilkinson A, LaLone CA. Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation. J Vis Exp. 2023 Feb 10;(192). doi: 10.3791/63970. PMID: 36847398.". This dataset is associated with the following publication: Vliet, S., M. Hazemi, D. Blatz, M. Jensen, S. Mayasich, T. Transue, C. Simmons, A. Wilkinson, and C. Lalone. Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation. Journal of Visualized Experiments. JoVE, Somerville, MA, USA, 192, (2023).