In zebrafish, 161 compounds were screened and 34 were identified by visual inspection as VDCs, of which 28 were confirmed as VDCs by quantitative image analysis. Testing of the zebrafish VDCs for their capacity to inhibit endothelial tube formation in the murine yolk-sac-derived endothelial cell line C166 identified 22 compounds that both disrupted zebrafish vascular development and murine endothelial in vitro tubulogenesis. This dataset is associated with the following publication: McCollum, C., J. Conde Vancells, C. Hans, M. Vazquez-Chantada, N. Kleinstreuer, T. Tal , T. Knudsen, S. Shah, F. Merchant, R. Finnell, J. Gustafsson, R. Cabrera, and M. Bondesson. (Reproductive Toxicology) Identification of vascular disruptor compounds by a tiered analysis in zebrafish embryos and mouse embryonic endothelial cells. REPRODUCTIVE TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 70: 60-69, (2017).