Data associated with "Wang N, Kunz JL, Hardesty DK, Steevens JA, Norberg-King T, Hammer EJ, Bauer CR, Augspurger T, Dunn S, Martinez D, Barnhart MC, Murray J, Bowersox M, Roberts J, Bringolf RB, Ratajczak R, Ciparis S, Cope WG, Buczek SB, Farrar D, May L, Garton M, Gillis PL, Bennett J, Salerno J, Hester B, Lockwood R, Tarr C, McIntyre D, Wardell J. Method Development for a Short-Term 7-Day Toxicity Test with Unionid Mussels. Environ Toxicol Chem. 2021 Dec;40(12):3392-3409. doi: 10.1002/etc.5225. Epub 2021 Nov 10. PMID: 34592004.". This dataset is associated with the following publication: Wang, N., J. Kunz, D. Hardesty, J. Steevens, T. Norberg-King, E. Hammer, C. Bauer, T. Augsberger, S. Dunn, D. Martinez, C. Barnhardt, J. Murray, M. Bowersox, J. Roberts, R. Bringolf,, R. Ratajczak, , S. Ciparis, W.G. Cope, S. Buczek, D. Farrar, L. May, M. Garton, P.L. Gillis, J. Bennett, J. Salerno, B. Hester, R. Lockwood, C. Tarr, D. McIntyre, and J. Wardell. Method Development for a Short-Term 7-d Toxicity Test with Unionid Mussels (RARE Project Development of Standard Methods for Two Freshwater Invertebrate Species, Mussels and Mayflies, for Whole Effluent Toxicity Testing and Receiving Waters). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(12): 3392-3409, (2021).