Measurements of water sulfate concentrations at the beginning and end of the exposures of the animals, initial δ(34S/32S) ratios in the animals at the beginning and end of the exposures, and initial δ(34S/32S) ratios of the exposure water. This dataset is associated with the following publication: Griffith, M., J. Lazorchak, and H. Haring. Uptake of Sulfate from Ambient Water by Freshwater Animals. WATER. MDPI AG, Basel, SWITZERLAND, 12(5): 1496, (2020).