This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), References: Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
A data delivery application that provides web-based access to of soil, water, climate, land management, and geospatial data produced by Conservation Effects Assessment Project (CEAP) watershed research sites across the United States. Data access via ArcGIS Server and MS SQL Server Enhanced data searches and summary options in Tools Access to high-resolution imagery in the Map>Table of Contents Enhanced graphing options on the Get Data page Transparency sliders for individual map components in the Map>Table of Contents
From the site: "Information on sediment-hosted zinc-lead deposits from around the world with grade and tonnage models, a general classification based on geologic setting, mineralogy, with data allowing locations of these deposits to be plotted using GIS."