The dataset contains fish mortality associated with various treatment of oil and dispersants in water accommodated fractions and sediment. The dataset also contains analytical chemistry associated with each experimental treatment. This dataset is associated with the following publications: Barron, M. Photoenhanced Toxicity of Petroleum to Aquatic Invertebrates and Fish. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY. Springer, New York, NY, USA, 73(1): 40-46, (2017). Barron, M., J. Kryzwa, C. Lilavois, and S. Raimondo. Photoenhanced toxicity of weathered crude oil in sediment and water to larval zebrafish. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY. Springer, New York, NY, USA, 100(1): 49-53, (2018).