This dataset contains files reconstructing single-cell data presented in 'Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing' by Herrera-Uribe & Wiarda et al. 2021. Samples of peripheral blood mononuclear cells (PBMCs) were collected from seven pigs and processed for single-cell RNA sequencing (scRNA-seq) in order to provide a reference annotation of porcine immune cell transcriptomics at enhanced, single-cell resolution. Analysis of single-cell data allowed identification of 36 cell clusters that were further classified into 13 cell types, including monocytes, dendritic cells, B cells, antibody-secreting cells, numerous populations of T cells, NK cells, and erythrocytes. Files may be used to reconstruct the data as presented in the manuscript, allowing for individual query by other users. Scripts for original data analysis are available at https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq. Raw data are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB43826. Funding for this dataset was also provided by NRSP8: National Animal Genome Research Program (https://www.nimss.org/projects/view/mrp/outline/18464).
- ZIPHerrera-Uribe & Wiarda et al. PBMCs - All Cells 10X Format
- ZIPHerrera-Uribe & Wiarda et al. PBMCs - All Cells H5Seurat
- CSVHerrera-Uribe & Wiarda et al. PBMCs - All Cells Metadata
- CSVHerrera-Uribe & Wiarda et al. PBMCs - All Cells PCA Coordinates
- CSVHerrera-Uribe & Wiarda et al. PBMCs - All Cells t-SNE Coordinates
- CSVHerrera-Uribe & Wiarda et al. PBMCs - All Cells UMAP Coordinates
- CSVHerrera-Uribe & Wiarda et al. PBMCs - CD4 T Cells t-SNE Coordinates
- CSVHerrera-Uribe & Wiarda et al. PBMCs - CD4 T Cells UMAP Coordinates
- CSVHerrera-Uribe & Wiarda et al. PBMCs - Gamma Delta T Cells UMAP Coordinates
- CSVHerrera-Uribe & Wiarda et al. PBMCs - Gamma Delta T Cells t-SNE Coordinates
- TXTHerrera-Uribe & Wiarda et al. PBMCs - Gene Annotation Information